Development of robust assays to measure 160+ proteins in blood and plasma









We developed a multiplex immunoassay for detection of 162 immune related proteins in human serum and plasma. The aim of this study was to evaluate precision, accuracy, reproducibility and stability of proteins when repeated freeze-thaw cycles are performed of this in-house developed panel, as well as assessing the protein signature in plasma and serum using various anticoagulants. Next we measured all mediators in serum, EDTA plasma and sodium heparin plasma of 43 healthy control donors. Principal component analysis revealed different coagulants cluster separately and that sodium heparin shows the most consistent profile. These results, with an extensive description of the methods and protocols are nog shared open access:

Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects.

Scholman RC, Giovannone B, Hiddingh S, Meerding JM, Malvar Fernandez B, van Dijk MEA, Tempelman MJ, Prakken BJ, de Jager W.

Cytokine. 2017 Oct 28. pii: S1043-4666(17)30336-8.