Welcome to the knowledge base of Ucan-U. Ucan-U wants to use this wiki to share relevant information related to its work. Currently Ucan-U only shares standard operating procedures in this system, but with your help we hope to be able to collect and organise a wealth of information in here.

 

Standard operating procedures

 

Flow cytometry

1. Immunofluorescent staining and flow cytometric analysis of cells
2. Immunofluorescent staining and flow cytometric analysis of human CD4+ Regulatory T cells
3. General Annexin V Staining Procedure
4. Proliferation of Human Mononuclear Cells with CFSE

 

Other

1. Detection of proteins of interest in cell lysates
2. Generation of EBV-immortalized B cell lines
3. Isolation of Human Mononuclear Cells from Blood
4. Isolation of Human Mononuclear Cells from Synovial Fluid
5. Lysis of Whole blood and Human Mononuclear Cells from Blood and Synovial Fluid
6. Plasma Isolation from Human Peripheral Blood
7. Proliferation of Human Mononuclear Cells with 3H-Thymidine
8. Serum Isolation from Human Peripheral Blood
9. Thawing Human Mononuclear Cells