Flow cytometry

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. Although these techniques are most widely used in mammalian biology, they can be applied to almost any particle with useful optical properties including bacteria, viruses, sub-cellular organelles and artificial particles, for example polystyrene beads bearing ligands or antibodies.

In most applications, after a particle exits the laser beam, it is sent to waste. Sorting allows us to capture and collect cells of interest for further analysis. Once collected, the cells can be analyzed microscopically, biochemically, or functionally.

Our mission is to provide comprehensive flow cytometric analysis and sorting including instrumentation, technical support, training and consultation.

 

 

A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves at reduced cost. A major part of our mission is to teach this technology to investigators, students, and staff. Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data.

 

Flow Cytometry Facility Instrumentation

Analytic Flow Cytometers

Currently we provide two flow cytometers for analysis work only.

  • BD FACSCanto™ II system that is available has 3 lasers for excitation (405nm, 488nm, and 635nm) providing up to 8 colors of fluorescence.
  • BD FACSCanto™ II system with BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition of  microtiter plates.  This system is also available with 3 lasers (405nm, 488nm, and 635nm)

 

Sorting Flow Cytometers

One sorting instrument is available.

  • BD FACSAria II cell sorter is a high speed cell sorter for measuring and sorting fluorescently labelled cells. FACSAria II cell sorter has 3 lasers for excitation (405nm, 488nm, and 635nm) providing up to 8 colors of fluorescence.

 

Contact

 

 

 

 

 

 

Wilco de jager

University Medical Center
Dept. of Pediatrics CMCI
KC01.069.0
Lundlaan 6
3484 EA Utrecht
The Netherlands
Phone +31 30 2503316
Fax +31 30 2503931

 


  
Login